Using a Micropipette

Delivery model with automatic Micropipette

Micropipette (microscopic nozzle) used to guide very small amounts of fluid infusion ( <1ml). Split level system used in the product this Micropipette is microliter – µl (1000 µ l = 1 ml). We use a Micropipette by Rainnin production and this product is also known as "Pipetman". This is a product with sophisticated design with relatively expensive, range 250$ – 300$ / 1 products. We have 4 types of dimensions are identified by the numbers on the circular button of pit-Tong. The main effect of this product is determined to be the maximum volume in microliters can be transferred with the size pipette. They are used along with sterile disposable plastic head. Here is the illustration of a micropipette:

Using a Micropipette

1. DO NOT exceed the permitted limit of the pipet.

The allowable limit as follows:

P10: 1.0 – 10.0 µl

P20: 2.0 – 20.0 µl

P200: 20 – 200 µl

P1000: 200 – 1000 µl

Look at the front of the pipet, you will see a blank box with 3 the figure is inside it. Below is a chart to help you can find the maximum values are the norm in each a different size of the pipet. Exceeding these values means pipet calibration is out already. In addition, next to each box below are the numbers that it represents. Therefore, Please take the time to read them to avoid damaging the pipet when dialing in excess of the permitted range of it.

The following illustrations help you read exactly the volume recorded on the micropipette:

P1000		P200 or P100		P20
1	1000' s	2,1	100' s	2	10' s
0	100' s	0	10' s	0	1' s
0	10' s	0	1' s	0	1/10ths

 

2. The size of the micropipette is suitable for your work ?

GENERAL RULES: Always select the SMALLEST size to pipet to your desired volume level . This will help you achieve the highest possible accuracy. Please note that the accuracy will decrease when you use the large size not pipet needed to norm 1 small volume.

3. Set the desired volume by twisting the round button in the center according to the clockwise if you want to increase the volume or counterclockwise turn clockwise if you want to reduce the volume.

P10: Maximum volume: 10 µl. Micropipette will norm be correctly when the volume range 1 µl- 10 µl. Indicator appears on the micropipette (There are usually black-Black-Red) be read as XX. X µ l. The change in color indicates the decimal part.

P20: Maximum volume: 20 µl. Micropipette will norm be correctly when the volume range 2 µl- 20 µl. Indicator appears on the micropipette (There are usually black-Black-Red) be read as XX. X µ l. The change in color indicates the decimal part.

P200: Maximum volume: 200 µl. Micropipette will norm be correctly when the volume range 20 µl- 200 µl. Indicator appears on the micropipette ( often there are 1 coloring) be read as XXX µ l.

P1000: Maximum volume: 1000 µl (= 1 ml). Micropipette will norm be correctly when the volume range 200 µl- 1000 µl. Indicator appears on the micropipette ( usually have red – Black – Black) be read as X. XX ml. Please note that this number is only read by ml micropipette while the remaining types read according to µ l.

4. Load the sterile tip top: Use the blue tip pipet tip clean and P1000 for for P200 and the entire pipette has smaller size. Use a filter when using the PCR or RNA. Remember to close the lid after use to maintain sterile properties.
   ATTENTION: Not allow the pipette tip top tap any object would (including gloves, clothing, the skin or hair).
5. Model cargo

Pit-Tong will stop in 2 different points when it is compressed down. Push pit- real tones slowly down the location of the first resistor: This is the volume of downloads. Because the first stops depends on the volume to be tv guide, Therefore, the distance to push pit-tribe reaches first resistance will change depending on the volume are the norm.

While keeping pit-Tong at the latest mass downloads, latest top tip on in the solution so that it is embedded 1 making just enough covering the end of the (3-4mm) , not the first tip may deepest dip.

From the drop-out to pit from the obtained liquid volume, ensure that still keep the head tip is embedded in the solution.

ATTENTION: If the solution that ban are the norm in the form of make, Let the top tip of the pipette to the largest volume dipped before the withdrawal early tip out to avoid appearing bubble and leading to the determination of the volume is not exactly.

Check again to be sure of the accuracy, There should be no vacuum at the end of the pit-Tong.

6. Provides sample: The second stops can be found when pit-compressed tones down beyond the first contact point until it is in contact with the body of the pipette. The second stop was used to complete the discharge of aqueous effluent off the top plastic. You should not reach this second stops when currency solution into the pipet, unless push out the last drops.

To convert the volume, need:

• Latest top tip on comments received
• Compress the pit-cardboard down the first contact point
• Wait 1 seconds
• Continue compressing the pit- tone down the bottom-it will discharged the entire liquid.
• In the end, withdraw the head tip that does not draw pitted-out tone.

7. Remove the top tip: While holding the top of the tip in the jar rather than proper waste, push the slider switch at the rear of the bath grip.

ATTENTION:

• Never been driven pipet up above. This makes the liquid flowing down to the bottom of the pipet and pipet damage.
• When withdrawing liquid out of the pipet, Remember to drop pit-Tong 1 the way from from. This prevents the liquid from being rushed into the end of the pipet and do it is blocked. This is especially important for large size pipet (200-1000 µl)
• Make sure to use the correct size of the top tip for each of the pipet.
• Always remember to use a new tip for each kind of different liquids.
• Using the pipet is suitable for the volume should be the norm. Do not ever use the pipet 200-1000 µ l to volume limit under 200 µl. Quota volume beyond the scope of the micropipette (too high or too low) all can cause damage to the device.

The technique for small volume

For small volume, especially in the range 1-10 µl, you need to keep track of each small drop as the norm.

Especially be careful removing each small drop in on of the test tubes so you can see it, withdraw the head tip out 1 carefully before removing the pit-Tong.

If the added 1 larger quantities, need to flush 1 liquid solvents for the early tip after removing the small drop to ensure that you get the whole liquid was the norm. With a small amount then you often have to centrifuge and then swirling test tubes to get the perfect mixture of reactants.

Simple test for correct calibration

Check the calibration of micropipette by using 1 ml of water has a mass of 1 g is the reduction of the ion or distilled. Quota volume range is from range use of pipette . Pipette errors >5% should be edited again.