Haemocytometer counting Chamber – Neubauer Improved

Haemocytometer counting Chamber Neubauer-improved

Common applications are in the hospital, biotechnology ... aims to determine the number of cells.

Haemocytometer counting Chamber classification often have 2 type

• The Chamber had a silver-coated cell count ( be more readable)
• Non coated silver cell counting Chamber

Autoclaves medical instruments

Guide to using haemocytometer counting Chamber Neubauer-improved

Step 1: Mao blood with norm 0,5 ml on the Micropipet (have white or pink balls beads), escape to the air entered in the blood, and non-aqueous diluent ( contains lazaruz or Marcano) use your finger under the top tube tube. Then the straw was placed horizontally, use your shake or hand-shake for the play

Methods for manual blood cell counting

The current method is also commonly used in many hospital Vietnam:

The principle of the taking of blood:

• Avoid mealtimes and patients not campaigning strongly to avoid Leukocytosis Physiology after meals, due to the many active.
• So on a fixed time to compare with previous checks for accuracy.
• Normally should get the blood in the capillaries in the fingers or retrieved in the earlobes for children.
• Antiseptic place defined by alcoholic magnet and wipe dry with clean cotton.
• Use magnet sterilizing needles. Deep enough to prod the blood should not squeeze the hands. Put the first drops of blood, take the second drop of blood ... If the hand squeeze, in the organizations can reconcile each other and blood thinning, wrong results.
• Dilute the blood cells are in the blood with a extremely large amount, If not dilute the blood to leave one by one then can't count.

Laboratory furnaces Naberther Germany

The solution to the usual dilution:

Erythrocytes: commonly used aqueous Hayem, Marcano, Gowers.

• Aqueous Hayem: include sodium sulfate, sodium chlorur, Mercury II chlorur and distilled water.
• Aqueous Marcano: include sodium sulfate, formol and distilled water.
• Aqueous Gowers: include sodium sulfate, acetic acid, and distilled water.

Leukemia: aqueous acetic acid include lazarut, methylen blue and distilled water. The Lazarus effect solution melt Hong requested on the microscope easy to watch over.

The laboratory used straws Potain to dilute the blood; There 2 private type used for leukemia or erythrocytes.

Red straws, columns-small, elected to great mix, in it the seeds of green or red glass to mix the blood, on the straws have carved the number 1; 0,5; 101: dilution levels 1/100 or 1/200.

Tube-white, columns-to, elected in small mixer, White glass beads. On the straws also carved the number 1; 0,5 but the top line is 11: dilution levels is 1/10 or 1/20.

The blood will be sucked into the tube to a step of Potain 1 or 0,5 According to predetermined dilution levels. Then-aqueous Marcano ... up to the step 101 with red straws and aqueous Lazarus up to step 11 for tube-white. Work fast so the clotting, shake thoroughly in 3 minutes to be dispersed

Hoods toxic gas the laboratory

Count blood cells using haemocytometer counting Chamber

The laboratory used the blood to a blood cell count. There are many blood type design: Goriaev, Agasse-Lafont, Fiessinger, Thomas, Levy, Neubauer, Malassez. These design blood is the thick lamella, between a concave, in many alternate horizontal lines along the divide into small squares.

• Neubauer has designer blood 25 squares to, each box has square area 0,04 mm2, the volume 1/250 mm3 and divided 16 small box. The volume of both 25 big square box is 1/10 mm3 and each small box is 1/4000 MM3.
• Blood Goriev design is similar to Neubauer but placement of the square has a different.
• Remove these drops top in straws, just get the drops in the mix, young blood into the next and then apply a glass leaf up, then bring up the microscope counting.

Counting each small box a, avoid counting count the erythrocytes is located in between 2 the box.
Count the many boxes and then take the average number (often must take to 80 small box news 5 large squares in the corners).
Multiplied by the number of fields in the 1 MM3 (= 1 µl) the blood and then multiplied with the dilution, We have a number of erythrocytes in 1 µl blood.
For leukemia also conducted such a large box in the count but (If the blood taking Neubauer design: count in 4 the area of the corner) because of the rate of leukemia is very little in comparison with erythrocytes

Causes for blood cell count not correct:

• Due to the tool: the tool when producing inaccuracies, tools for long wear and tear, or washing the dirty left his blood was no more.
  Due to the technical: When many fingers squeeze back blood lymph and blood work; not enough blood volume regulation, shake the irregular ...
  Due to technical staff: familiar or unfamiliar do, eye strain when doing too much, the method count inconsistency.

In general these reasons on the error is ± 3% can be considered low for.

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