Environment of Tetrathionate Broth Base Titan Novobiocen Scott Mueller

Environment of Tetrathionate Broth Base Titan Novobiocen Scott Mueller

Used in the selective enrichment and isolated Salmonella bacteria

Components of Tetrathionate Broth Base Titan Novobiocen Scott Mueller

Hóa chất vi sinh natural hygroscopic powder, should be preserved in dry place, in closed under temperature 25 degrees C and avoid direct light.

Guide to using Tetrathionate Broth Base Titan Novobiocen Scott Mueller

Dissolve 89.4 g of distilled water 1000 ml. To soak 10 minutes, Stir to mix and heat slowly to boiling. Cannot be sterilized, then come cooler 45 C to use.

Add 20 ml solution of Iodine and a bottle of MKTT Novobiocin supplement-TS152. Mix well and let in the glass test tubes sterile.

How to mix use of Iodine: Dissolve 25 grams of Potassium Iodide in 10 ml of water. Add 20 g iodine and diluted into 100 ml with water desalination ion sterilization already.

Attention: Due to the presence of chemicals CaCO3, medium white precipitate. If you need to, adjust the pH to the desired value.

Good chemical signs: Light opalescent Green. The pH at 25 degrees C: 8.2 ± 0,2

Principle of Tetrathionate Broth Base Novobiocen Scott Mueller

Use to get rich and isolated Salmonella bacteria in food and animal feed. Microbial environment is used first by the microorganism Muller in 1923 in order to inhibit Fecal Coliform bacteria. The environment contains Sodium Thiosulphat have the task of creating tetrathionat by adding iodine to the biotechnology environment.

Tetrathionate prevents the development of Coliform and other substances of intestinal bacteria. Sodium chloride release electrolytes necessary for the transport and osmotic balance. Calcium carbonate to neutralize sulfuric acid environment when Tatrathionat reduced. Brilliant Green, Ox Bile and Novobiocin provide ingredients that inhibit the growth of Gram-positive bacteria of the other except bacteria Salmonella.

See also the chemicals Salmonella cultured environment