Medium rappaport vassiliadis broth enrichment of salmonella
Used in the enriched selective Salmonella under osmotic controls high and low pH
Chemical composition of Rappaport Vassiliadis environment Salmonella Broth Enrichent Titan
Chemical composition |
Chemical content |
Magensium chloride (hexahydrate) |
29.00 |
Sodium chloride |
8.00 |
Soya peptone |
4.50 |
Potassium phosphate, monobasic |
0.600 |
Dipotassium phosphate |
0.40 |
Malachite green |
0.036 |
This is Salmonella cultured environment natural hygroscopic powder, should be preserved in dry place, in closed under temperature 25 degrees C and avoid direct light.
How to use Rappaport Vassiliadis environment Salmonella Broth Enrichent Titan
User Weight techniques 2 number of travellers, 27.10 GMS dissolved in 1000 ml distilled water. Use Air stirring from his heat heat from boiling and stir the light from coming in until completely melted. Distribute 10 ml into each glass test tubes having the cap nuts. Sterilization in 115 degrees C within 15 minutes and cooling of environment 25 degrees C
Good chemical signs: Blue turquoise to green, in the slightly opaque white, Ph (at 25° C): 5.2 ± 0.2
The principle of operation of the Rappaport Vassiliadis environment Salmonella Broth Enrichent Titan
Using selective enrichment to Salmonella bacteria. Development environment component is "Rappaport" capable against the larger Hyperton cells the rest of gut bacteria. The British experiments He found that Magnesium Chloride Rappaport is best understood in the salt use.
Then, two researchers Schothorst and Rappaport Vassiliadis Broth amendment Renaud by substituting Casein Peptone Peptone soy into doing the carbon nutrition source, nitrogen for growth needs. Combining more chemicals Nacl, Potassium photphate and Di potassium phosphate to form a more stable formula for the environment over time.
Hybrid environment with Malachite Green has green aims to inhibit microorganisms other than Salmonella Spp.
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