How to calibrate and check the Micropipet automatically

1. Micropipet test lab
   

There are now many checking the micropipet as the method of density, spectral methods, immune method or using automated test machine. However the most popular way currently applied is that density method. I.e. use the correct weight to determine the weight of the volume of water was sucked by micropipet from which determine the actual volume has-of installation volume compared to pipet.

To check be Micropipet you first need to know the requirements to check that is: With the type of Electronic Micropipet can a fixed area, then you need to check 10 at times the volume it. With the pipet can change the volume to check in 3 the point is the point of greatest volume, the most compact point, the average score at each point and you also need to check the 10 visit . For example, you may type -1000 100 µ l pipet then you need to check in 3 the score is 1000 µ l, 1000µ l and 500 µ l and each point you make repeat 10 visit .

To test you need to prepare the following instrument types:

• Distilled water 2 visit .
• Grains have mine 50, 100ml.
• Electronic Scales 4 the number of travellers with an accuracy of 0, 0001g used to check the type of pipet can area of greater than 100 µ l. Weight 5 numbers with an accuracy of 0, 00001g is used to check the pipet volumes greater than 10 µ l. Weight 6 numbers with an accuracy of 0, 000001g is used to check the volume less than or equal to 10 µ l. Type 6 This little number has laboratories because of its very high price. So the fact to check what type of pipet can this small area often are sent to 1 independent test unit.
• There is room temperature stable, It is best put in the room has air-conditioning and installation of desired temperature.
• Standard thermometers: Used to measure the temperature of the water.

Put the entire laboratory instruments, water, the thermometer in the room to stabilize temperature. At least 30 minutes before proceeding.

After you have prepared as above you will conduct the following checks:

• Turn the knob to adjust the volume by volume pipet to check.
• Retrieved 1 Cup 50 ml mine, Add 5 ml of distilled water into the, put on weight and adjust the weight value on "O".
• The first con in Mount pipet, the first con-coated 3 times with distilled water.
• -Distilled water using the pipet then drop into the Cup have mine on balance analytical. Write the mass balance which determines.
• Do repeat 10 visit . Notes the results back.

Can interest you : Medical autoclaves

After you have had the results you will proceed to the calculation to determine the true and chụm.

Check chụm:

You calculate the average value of 10 measurements are then calculated relative standard deviation RSD% (or CV%). This relative standard deviation must be no larger than the values in the following table:

• With that kind of fixed volumetric pipet:

Type pipet – µl	Speed chụm – CV%
10	< 0,5
25	< 0,3
50	< 0,3
100	< 0,2
200	≤ 0,2
500	≤ 0,2
1000	≤ 0,2

• With that kind of volume change pipet:

Type pipet – µl	Volume points – µl	Speed chụm – CV %
1-10	1	≤ 2,8
1-10	5	≤ 0,8
1-10	10	≤ 0.4
2-20	2	≤ 1,5
2-20	10	≤ 0,6
2-20	20	≤ 0.3
5-50	5	≤ 1,5
5-50	25	≤ 0,3
5-50	50	≤ 0,3
10-100	10	≤ 0,7
10-100	50	≤ 0,3
10-100	100	≤ 0,2
20-200	20	≤ 0,7
20-200	100	≤ 0,3
20-200	200	≤ 0,2
100-1000	100	≤ 0,4
100-1000	500	≤ 0,3
100-1000	1000	≤ 0,2

Check the correct:

First of all to check properly you need to determine the actual volume of pipet by converting from mass has weight is according to the following formula:

V = m/D. Where V is the volume of food that need identifying, m is the mass of water has weight is, D is the density of water in conditions of temperature and 1atm pressure measurement. Each different temperature water will have different density. You see the table below to determine the density corresponding to the temperature:

Temperature (°C)	The proportion of the (mg/l µ)
0,0	0,9998425
4,0	0,9999750
10,0	0,9997026
15,0	0,9991026
17,0	0,9987779
20,0	0,9982071
25,0	0,9970479
37,0	0,9933316
100	0,95803665

 

After you have identified the correct volume per weight at the corresponding temperature you conduct computer degrees according to the following formula:

Degrees true (%) = (V-Vo)/Vo x 100

In that: V is the volume of food are. Vo is the volume under the theory

For example you-500 µ l of water in 20 C weight was 502 How to calculate the correct level then the mg as follows:

Real v = m/D = 502/0,9982071 = 502.9 µl

-> Degrees true(%) = (502,9-500)/500 x100 = 0,58%.

Now we see the results has hit or not by contrast with the table of correct altitude values allowed as follows:

With the pipet can earn fixed:

Micropipet type – µl	Degrees true – %
10	± 1.2
25	± 1.0
50	± 0.7
100	± 0.6
200	± 0.6
500	± 0.6
1000	± 0.6

With the type of pipet not fixed volume:

Type pipet- µl	The point volume-µl	Degrees true – %
0,5-10	1	± 5.0
0,5-10	5	± 2.5
0,5-10	10	± 1.5
2-20	2	± 5.0
2-20	10	± 1.0
2-20	20	± 0.8
5-50	5	± 2.5
5-50	25	± 0.7
5-50	50	± 0.7
10-100	10	± 2.5
10-100	50	± 0.8
10-100	100	± 0.8
20-200	20	± 2.5
20-200	100	± 1.0
20-200	200	± 0.6
100-1000	100	± 2.0
100-1000	500	± 1.0
100-1000	1000	± 0.6

Back to the example above, you see with the type of pipet 500 µ l you calculate true altitude is 0,58% compare with the table above we see this value in the allowable limit. So this right has reached pipet. However in the case do not reach the stars? At this time you will need to review pipet calibration. So how do the calibration again.

2. Pipet calibration

The calibration is done after you check the results beyond the threshold allowing. How to calibrate as follows:

First you define the volume needed to calibrate using the following formula:

Δ V = (V – Vo)

In that: Δ V is the volume needed to calibrate. V is the actual value of the pipet to calculate the above. Vo is the volume of the pipet.

For example pipet 500 µ l after checking the actual volume of 510 µ l so the volume needed to calibrate is 10 µ l.

We will proceed to calibrate using the pipet carefully screw in the Cle quay to position the piston need to calibrate. A rotating cycle corresponding to the volume needs adjustment as the following table:

With that kind of fixed volumetric pipet:

Volumetric pipet – µl	The volume 1 cycle nuts – µl
10µ µ l or 20 l	0,8µl
25µ l or 50 µ l	4µl
100µl	4µl
200µ l or 250 µ l	38µl
500µ µ l or 1000 l	38µl

With the type of pipet not fixed volume:

Volumetric pipet – µl	The volume 1 cycle nuts – µl
10-100µl	5µl
20-200µl	10µl
100-100µl	50µl
500-5000µl	250µl
1-10ml	510µl

After editing is complete you should check the pipet as instruction how to check in on the Watch has not yet reached. If hit, and then you can use the pipet. If not you need to calibrate and check again until reaching.

This is a detailed guide on how to calibrate and check the micropipet in laboratory ! For further enquiries and purchase, please contact hotline of INNOTEC ! Please be understanding .