Protein analysis process according to the principle of the kjeldahl

Learn about the process of protein analysis according to the principle of the kjeldahl or kjeldahl systems in the laboratory – INNOTEC Vietnam

Process analysis of protein-protein in animal feed

1. Sample preparation

For about 100-200 gram sample of thank you, or sleep on the Cup grinding machine model ( speed round from 10.000 to 15.000 round) and crushing of hope 15 – 30 seconds, should note when using the grinders should not to contact operation screw marks too 15 seconds.

Then, give in to the 0.5 mm or smaller as possible. Use the zip bags to contain the template to avoid moisture.

2. Chemicals and tools required

Chemicals:

• Concentrated H2SO4 ( China ) do the environment breaking the pattern
• NaOH 30 -40% for store protein
• H3BO3 4% for store protein
• CuSO4 and K2SO4 chemicals break the link peptides in breaking the pattern, use the Se if needed ( toxic substances should not recommended use)
• Tasiro directive using the titration
• Standard solutions H2SO4 0.1 N the titration of Vietnam should quality for cheap ( Note brewing, because of this chemical influence to results)

The tool:

• Pipet titration 25 -50 ml should use of Germany
• Triangular vase 250 ml
• Pipe take off Kjeldahl nitrogen
• Electronic Scales 4 number of travellers ( use balance analytical Ohaus PA214 with reasonable price, If condition should use the electronic scales of Japan or Germany)
• Sample crushing machine
• Stainless steel screening 0.2 or 0.5 mm
• Machine Kjeldahl digestion
• -Protein distillers
• Cabinet air toxic or non-toxic equivalent when the device

3. Break the templates use the template kieldahl demolitions

Depending on the protein content of samples of high or low, volume broke the pattern may change from 0.5 grams to 5 gram.

Weight approx. 2 gram sample with the accuracy 4 number of travellers, for on the tube break model ( should wrap by wrapping paper patterns to avoid loss of samples or samples clinging to the tube).

Next for more about 5.5 CuSO catalyst 4.5 grams of H2O and K2SO4 with make 1:10 on the pipe break model. More about 15-20 ml of concentrated H2SO4 Axits on termite tubes.

That deep, for all pipe break this template on sample demolitions Kjeldahl , Heat up to 420 degrees C within 2 now ( When the form is in green are). Let it cool and let in distillation

4. Implement processes to analyze protein-protein distillers through automatically

Program settings the following distillation:

• The response time: 30 seconds
• Distillation time: 5 minutes
• The volume of NaOH of approximately 50 – 70 ml
• H2O volume range 10 ml
• Boric acid H3BO3 volume if available: 25 ml

5. The calculation results

• For about 2 drops of indicator on the average sample healing triangle
• Using the pipet and titration calculations are consuming 0.1 N H2SO4 volume (In)
• Then , apply the formula according to the standard

Free consultation procedure-protein analysis – protein by phone number: 0902106379

Above is the article on protein analysis process according to the principle of the kjeldahl or kjeldahl systems in the laboratory . Any questions about the article please contact INNOTEC in contact section – Sincerely thank – Best regards .

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