Environment Lactose TTC Agar with Heptadecyl Sodium Sulphate is used in the analysis of Ecoli and Coliform in water under standard TCVN 6187 – 1 : 2009 and ISO 9308 – 1 : 2000 According to the membrane filtration method.
The chemical composition of Lactose TTC Agar Titan
Chemical composition |
Chemical content. Gms/Ltr |
Lactose intolerance |
20.00 |
Agar |
15.00 |
Peptone |
10.00 |
Yeast extract |
6.00 |
Beef extract |
5.00 |
Heptadecyl sodium sulphate |
0.10 |
Bromothymol blue |
0.05 |
Package |
500g |
Hóa chất vi sinh TTC Agar lactose have Sodium Heptadecyl Sulphat natural hygroscopic powder, so the need to preserve a cool dry place, in peace and avoid direct light.
How to mix medium Lactose TTC Agar
Use two techniques to the odd weight dissolved in 1000 ml distilled water 15 g, 56. Heat slowly and stir until completely melted. Then, Sterilization by autoclave in 15 PSI (121 ° C) in 15 minutes. Cool to 45-50 º C and add 3ml solution of TTC 1% (TS 042). Shake lightly to assimilate and pour into sterile petri.
Good chemical identification: Pale green and in. PH meter (in 25 ° C): 7,2 ± 0,2 can use pH meter to manipulate.
The principle of micro-chemical use Lactose TTC Agar
Environment lactose TTC agar (with heptadyll sodium sulphate (According to ISO) be used to detect and quantify the bacteria e. coli and coliforms using the membrane filter technique according to international standard ISO 9308-1. Intermediate composition containing Peptone, Meat extracts and recurrent high mem to provide nutritional ingredients such as: nitrogen, carbon compounds, vitamins and amino acids. Lactose is sugar fermentation. Sodium
heptadecyl sulfate (Tergitol 7) helps to inhibit most Gram-positive bacteria. Bromothymol blue is a directive can create color and Ph. Master of Agar used to create the mold. This environment is brewed along the membrane filter in 36 ± 2 độ C và 44 ± 4 C during the period 21 ± 3 now.
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