Environmental Microbiology Lauryl Sulphate Broth TM150

Environmental Microbiology Lauryl Sulphate Broth be used in the determination of Coliform bacteria.

The chemical composition of Lauryl Sulphate Broth TM150

Môi trường nuôi cấy vi sinh in the form of dry powder, need to preserve in dry place in sealed box under temperature 25 degrees C and avoid direct light.

How to use processing and Lauryl Sulphate Broth TM150

Dissolve 35.6 gram in 1000 ml distilled water. Then, boil up from from and stir lightly to the completely soluble. You can use Air stirring from his heat to perform more quickly. Pour 10 ml has been dissolved into the glass test tubes having the CAP contains the Durham (Inverted reverse). Reduction of corporal by autoclaves at 15 PSI- 121 degrees C within 15 minutes. Then cooled to 45 -50 degrees C before use.

Good chemical signs: Pale yellow, in. And the pH in the 25 degrees C: 6.8 ± 0.2

The principle of the use of the micro-environment Lauryl Sulphate Broth TM150 Titan

LAURYL SULPHATE environment the BROTH used to detected coliform bacteria in the water and in food products such as: Candy, feed, the food .... Lauryl sulphate promoting the school and coliform microorganisms gas. Aerobic bacteria were inhibited completely. Component Tryptone to provide nutrients such as nitrogen compounds, carbon, vitamins and amino acids. Lactose is sugar fermentation.

Sodium chloride component aimed to maintain osmotic balance of the environment. Dipotassium phosphate and Mono potassium phosphate buffer component to control the pH in the fermentation of Lactose intolerance. Lactose-positive bacteria metabolize lactose into gas and inverted Durham tubes are collected in.

The environment was incubated at the appropriate temperature within 18-24 now. In the case generated gas, the Durham tube gas easily observed. The still opaque environment accompanied by the formation of gas within 48 now the positive assumption of test bacteria. coli or fecal coliform bacteria to another.

The environment after phase can be preserved in 2-8 o C, but need to be cleaned at room temperature. If any fermentation in the tube is incubated at 44 ° C after 8 to 24 now, indole test performed by adding reagent Kovac (TR 008). Positive indole test in a test tube can be observed due to gas in the 44 ° C, indicates the presence of the bacteria Escherichia coli. – Ecoli